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After incubation on the corresponding days, 10 µL of CCK-8 solution (#FC101-04, TransGen, Beijing, China) was added to each well and incubated at 37 °C for 2 h

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The fact that IGF1-SDS values increased significantly in our cohort, whereas IGFBP3-SDS values showed a trend toward increase, is consistent with the knowledge that IGF1 usually responds more markedly to stimuli than IGFBP3 [35]